Diseases of Aquatic Organisms | |
Inactivation of infectious salmon anaemia virus, viral haemorrhagic septicaemia virus and infectious pancreatic necrosis virusin water using UVC irradiation | |
Espen Rimstad1  Ann Kristin Øye1  | |
关键词: UVC inactivation; Infectious salmon anaemia virus; Infectious pancreatic necrosis virus; Viral hemorrhagic septicaemia virus; | |
DOI : 10.3354/dao048001 | |
学科分类:生物科学(综合) | |
来源: Inter-Research | |
【 摘 要 】
ABSTRACT: The UVC irradiation doses necessary for a 99.9% (3-log) inactivation of 3 different fish pathogenic viruses diluted in freshwater/seawater and wastewater from a fish processing plant were determined. The results showed that both infectioussalmon anaemia virus (ISAV) and viral haemorrhagic septicaemia virus (VHSV) were very sensitive to UVC irradiation, showing a 3-log reduction of infectivity in freshwater of 33 ± 3.5 and 7.9 ± 1.5 J m-2, respectively, while that of infectiouspancreatic necrosis virus (IPNV) was substantially higher, 1188 ± 57 J m-2. Using ISAV as a model, a comparison of the effect of UVC irradiation on virus isolation versus reverse transcription polymerase chain reaction (RT-PCR) showed thatconsiderably higher UVC doses, depending on the length of the amplified product, were necessary to abolish RT-PCR detection of viral RNA.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201911300208291ZK.pdf | 87KB | download |