Inactivation of infectious salmon anaemia virus, viral haemorrhagic septicaemia virus and infectious pancreatic necrosis virusin water using UVC irradiation

Diseases of Aquatic Organisms

Espen Rimstad¹  Ann Kristin Øye¹ 

关键词: UVC inactivation;    Infectious salmon anaemia virus;    Infectious pancreatic necrosis virus;    Viral hemorrhagic septicaemia virus;   

DOI  :  10.3354/dao048001

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The UVC irradiation doses necessary for a 99.9% (3-log) inactivation of 3 different fish pathogenic viruses diluted in freshwater/seawater and wastewater from a fish processing plant were determined. The results showed that both infectioussalmon anaemia virus (ISAV) and viral haemorrhagic septicaemia virus (VHSV) were very sensitive to UVC irradiation, showing a 3-log reduction of infectivity in freshwater of 33 ± 3.5 and 7.9 ± 1.5 J m^-2, respectively, while that of infectiouspancreatic necrosis virus (IPNV) was substantially higher, 1188 ± 57 J m^-2. Using ISAV as a model, a comparison of the effect of UVC irradiation on virus isolation versus reverse transcription polymerase chain reaction (RT-PCR) showed thatconsiderably higher UVC doses, depending on the length of the amplified product, were necessary to abolish RT-PCR detection of viral RNA.

【 授权许可】

Unknown