First isolation of an aquatic birnavirus from farmed and wild fish species in Australia

Diseases of Aquatic Organisms

Allan Gould¹  NicholasGudkovs¹  Judith Handlinger¹  Lauretta M. Eaton¹  Mark St. J. Crane¹  Jackie Kattenbelt¹  Alex D. Hyatt¹  , Lynette M. Williams¹  Paul Hardy-Smith¹ 

关键词: Virus isolation;    Aquatic birnavirus;    Farmed fish;    Wild fish;    Australia;    Infectious pancreatic necrosis;    IPNV;    PCR;   

DOI  :  10.3354/dao043001

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: During routine sampling and testing, as part of a systematic surveillance program (the Tasmanian Salmonid Health Surveillance Program), an aquatic birnavirus was isolated from Œpinhead¹ (fish exhibiting deficient acclimatisation on transfer tosaltwater) Atlantic salmon Salmo salar, approximately 18 mo old, farmed in net-pens located in Macquarie Harbour on the west coast of Tasmania, Australia. The isolate grows readily in a range of fish cell lines including CHSE-214, RTG-2 and BF-2and is neutralised by a pan-specific rabbit antiserum raised against infectious pancreatic necrosis virus (IPNV) Ab strain and by a commercial pan-specific IPNV-neutralising monoclonal antibody. Presence of the virus was not associated with gross clinicalsigns. Histopathological examination revealed a range of lesions particularly in pancreatic tissue. The virus was localised in pancreas sections by immunoperoxidase staining using the polyclonal antiserum and by electron microscopy. Examination byelectron microscopy demonstrated that the virus isolated in cell culture (1) belongs to the family Birnaviridae, genus Aquabirnaviridae; (2) was ultrastructurally and antigenically similar to virus identified in the index fish; (3) is related toIPNV. Western blot analysis using the polyclonal rabbit antiserum confirmed the cross-reactions between various aquatic birnavirus isolates. In addition, PCR analysis of isolated viral nucleic acid from the index case indicated that the virus is moreclosely related to IPNV fr21 and N1 isolates than to other birnavirus isolates available for comparison. Sampling of other fish species within Macquarie Harbour has demonstrated that the virus is present in several other species of fish including farmedrainbow trout Oncorhynchus mykiss, wild flounder Rhombosolea tapirina, cod Pseudophycis sp., spiked dogfish Squalus megalops and ling Genypterus blacodes.

【 授权许可】

Unknown