Large-Scale Survey of Mitochondrial D-Loop of the Red-Crowned Crane Grus japonensis in Hokkaido, Japan by Convenient Genotyping Method

Journal of Veterinary Medical Science

Akira SHIOMI²  Takeo HIRAGA²  Hiroki TERAOKA²  Hiroyuki MASATOMI¹  Mitsuhiko ASAKAWA²  Kunikazu MOMOSE¹  Takio KITAZAWA²  Junya SHIRAISHI²  Yoshiaki MIURA²  Kohei MAKITA²  Yulia MOMOSE¹ 

[1] NPO Tancho Protection Group, Kushiro 085–0036, Japan;School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan

关键词: Grus japonensis;    Hokkaido;    Japan;    mitochondria;    Red-crowned Crane;   

DOI  :  10.1292/jvms.12-0193

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(14)Cited-By(5)The Red-crowned Crane, Grus japonensis, is an endangered species of crane that has two separate breeding populations, one in the Amur River basin (continental population) and the other in eastern or northern Hokkaido, Japan (island population). So far, only two haplotypes (Gj1 and Gj2) have been identified in the mitochondrial D-loop in island population, whereas seven haplotypes have been found in continental population (Gj3–Gj9). We developed a rapid and inexpensive method of extensive genotyping of D-loop haplotypes in Red-crowned Cranes, based on amplification refractory mutation system (ARMS) PCR assay. Two hundred and three cranes in eastern Hokkaido were studied with this method and supplemental DNA sequencing. Only two haplotypes, Gj1 and Gj2, were confirmed in eastern Hokkaido with Gj2 as a major haplotype. Additionally, only Gj2 was identified in twelve feathers from both sexes found in northern Hokkaido. These results suggest scarce genetic diversity in island population of Red-crowned Cranes in Hokkaido, Japan.

【 授权许可】

Unknown