ISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTS | Science Publications

OnLine Journal of Biological Sciences

Guetarni Hassina¹  Bensoltane Ahmed¹ 

关键词: Helicobacter Pylori;    Culture;    Urease;    Real-Time PCR;    Clarithromycin;   

DOI  :  10.3844/ojbsci.2013.41.49

学科分类：生物科学（综合）

来源: Science Publications

【 摘 要 】

The objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from homogenates of gastric biopsies, quantification by real-time PCR was performed to determine Helicobacter pylori on the one hand and sensitivity to clarithromycin species. Melting curve analyzes showed that these two strains are sensitive to the antibiotic.

【 授权许可】

Unknown