期刊论文详细信息
OnLine Journal of Biological Sciences | |
ISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTS | Science Publications | |
Guetarni Hassina1  Bensoltane Ahmed1  | |
关键词: Helicobacter Pylori; Culture; Urease; Real-Time PCR; Clarithromycin; | |
DOI : 10.3844/ojbsci.2013.41.49 | |
学科分类:生物科学(综合) | |
来源: Science Publications | |
【 摘 要 】
The objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from homogenates of gastric biopsies, quantification by real-time PCR was performed to determine Helicobacter pylori on the one hand and sensitivity to clarithromycin species. Melting curve analyzes showed that these two strains are sensitive to the antibiotic.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201911300201314ZK.pdf | 199KB | download |