Differentiation of Foot-and-Mouth Disease Virus-Infected Pigs from Vaccinated Pigs using a Western Blotting Assay Based on Baculovirus-Expressed Nonstructural Proteins 2C and 3D

Journal of Veterinary Medical Science

Katsuhiko FUKAI¹  Seiichi OHASHI¹  Kazuo YOSHIDA¹  Kazuki MORIOKA¹  Kenichi SAKAMOTO¹  Reiko YAMAZOE¹ 

[1] Exotic Diseases Research Station, National Institute of Animal Health

关键词: baculovirus;    foot-and-mouth disease virus;    nonstructural protein;    swine;    western blotting;   

DOI  :  10.1292/jvms.70.1353

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(2)Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural proteins 2C and 3D were used as the antigens in a western blotting assay. This assay allowed for differentiation of FMDV-infected pigs from vaccinated pigs. Serial studies were performed using sera collected from experimentally infected and vaccinated pigs. Positive reactions were found in the sera derived from the experimentally infected pigs. There was, however, no positive reaction in the vaccinated pigs. These results suggest that this western blotting assay is a useful method for differentiation of infected pigs from vaccinated pigs.

【 授权许可】

Unknown