Analysis of Serum Cytokine Profile in a Holstein Heifer with Leukocyte Adhesion Deficiency Which Survived for Long Period

Journal of Veterinary Medical Science

Katsuro HAGIWARA¹  Rikio KIRISAWA¹  Hajime NAGAHATA²  Hiroshi IWAI¹  Hidetoshi HIGUCHI² 

[1] Department of Veterinary Microbiology, School of Veterinary Medicine, Rakuno Gakuen University;Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University

关键词: BLAD;    IL-1b;    IL-6;    IL-8;   

DOI  :  10.1292/jvms.64.683

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Cited-By(3)Serum cytokine levels and their expression of mRNA on neutrophils from a bone marrow (BM) transplanted heifer with leukocyte adhesion deficiency were evaluated. The clinical condition of the affected heifer was relatively stable after BM-transplantation. Persistent hyper γ-globulinemia and increased serum immunoglobulin G (IgG) concentrations were monitored longitudinally. The concentration of interleukin (IL)-1β in serum from the affected heifer ranged from 15.8 to 321.7 ng/ml, and maximum concentration occurred at the time which coincided with peak IL-6. Serum levels of IL-6 ranged from 0.32 to 27.9 ng/m l, and they appeared to be associated with the increment of serum IgG in the affected heifer. mRNAs for IL-1β, IL-6, IL-8 and granulocyte and macrophage colony stimulating factor (GM-CSF) were increased in neutrophils from the affected heifer compared to controls. Persistent hyper γ-globulinemia of the affected heifer appeared to be associated with enhanced mRNA expression for IL-6 and its serum levels. These findings suggest that humoral immunity of the affected heifer is activated and the production of neutrophils appears to be enhanced under the incapability of β2 integrin-mediated functions of phagocytic cells.

【 授权许可】

Unknown