PCR-Based Detection of Leishmania donovani DNA in a Stray Dog from a Visceral Leishmaniasis Endemic Focus in Bangladesh

Journal of Veterinary Medical Science

Mohammad Zahangir ALAM²  Hirotomo KATO²  Ken KATAKURA²  Md. Golam YASIN¹  Tatsuya SAKURAI² 

[1] Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh–2202, Bangladesh;Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, Sapporo 060–0818, Japan

关键词: Bangladesh;    canine;    Leishmania donovani;    PCR;    visceral leishmaniasis;   

DOI  :  10.1292/jvms.12-0134

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(3)Although Phlebotomus argentipes as the only known vector of visceral leishmaniasis (VL) is zoophilic in nature, VL is considered to be anthroponotic in the Indian subcontinent. Peripheral blood samples from 85 stray dogs were examined for any molecular evidence of Leishmania infection in VL endemic areas of Bangladesh. Parasite DNA was detected in a blood sample from 1 of 85 (1.2%) stray dogs using ITS1-PCR, and PCR sequencing of the rRNA-ITS and cytochrome b gene confirmed that the parasitic DNA was Leishmania donovani. The results support the assumption that dogs are a probable animal reservoir for the Leishmania parasite in Bangladesh. It will be important to investigate the possible epidemiological role of dogs in domestic foci of VL endemic areas in Bangladesh.

【 授权许可】

Unknown