Evaluation of a recombinant p24 antigen for the detection of Feline Immunodeficiency Virus-specific antibodies

Pesquisa Veterinária Brasileira

Leite, Romulo C¹  Reis, Jenner K.P¹  Danelli, Maria das Graças M²  Góes, Ana Carolina M.A. de⁴  Medeiros, Marcos A⁴  Hagiwara, Mitika K³  Mazur, Carlos² 

[1] Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;Universidade Federal Rural do Rio de Janeiro (UFRRJ), Brazil;Universidade de São Paulo (USP), São Paulo, Brazil;Fundação Oswaldo Cruz, Rio de Janeiro, Brazil

关键词: FIV;    feline immunodeficiency virus;    cats.;   

DOI  :  10.1590/S0100-736X2010001000011

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

Feline Immunodeficiency Virus is a worldwide infection and is considered a significant pathogen. The diagnosis of FIV infections is mainly based on commercially available rapid tests that are highly expensive in Brazil, hence it is rarely performed in the country. Furthermore, lentiviruses grow slowly and poorly in tissue cultures, making the production of viral antigen by classic means and thus the establishment of FIV immunodiagnosis impracticable. In order to deal with this, recombinant DNA techniques were adopted to produce the protein p24, a viral capsid antigen. The protein's reactivity evaluation analyzed by Western blot indicated that this recombinant antigen can be a useful tool for the immunodiagnostic of FIV infections.INDEX

【 授权许可】

CC BY-NC