Novel single nucleotide polymorphisms in candidate genes for growth in tilapia ( Oreochromis niloticus )

Revista Brasileira de Zootecnia

Rodríguez-González, Hervey²  Ambriz-Morales, Pascuala¹  Cuevas-Rodríguez, Breidy Lizeth²  Valdez-González, Francisco Javier²  Sifuentes-Rincón, Ana María¹  García-Ulloa, Manuel² 

[1] Instituto Politécnico Nacional, Reynosa, Mexico;Instituto Politécnico Nacional, Guasave, Mexico

关键词:  assisted selection markers;    genotypic frequency;    growth hormone gene;    myogenin geneIntroductionDetermination of the genetic variability is an essential step for the implementation of genetic improvement programs focused on the selection of faster growing fish with lower feed conversion rates and resistant to diseases (Lupchinski Jr;   

DOI  :  10.1590/S1806-92902016000600009

学科分类：兽医学

来源: Universidade Federal de Vicosa

【 摘 要 】

The objective of the present work was to identify and validate single nucleotide variations located in candidate genes to growth traits in tilapia (Oreochromis niloticus). Two transitions were identified in the promoter region of the growth hormone gene (GH); eight nucleotide changes were identified in introns and promoter region of the IGF-I gene; and a transition (T/C) was identified in the Myogenin gene (MyoG). The highest genotypic frequency (0.8) for GHpA1 and MyoG was found in the GG and TT homozygous individuals, while the highest frequency (0.9) for GHpB1 was observed in the CT heterozygous fish. There was no genotypic frequency in the CC homozygous tilapia for the GHpB1 and MyoG markers. Based on their allelic frequencies, validation as novel single nucleotide polymorphisms (SNP) of those variations located at O. niloticus GH and MyoG genes was possible. These new markers will allow their association with growth traits in tilapia to be exploited in order to determine their potential use as assisted-selection markers.

【 授权许可】

Unknown