Inhibitory effects of trichostatin A on adrenocorticotropic hormone production and proliferation of corticotroph tumor AtT-20 cells

Endocrine Journal

Yuko Asari¹  Aya Sugiyama¹  Yasumasa Iwasaki²  Kanako Niioka¹  Makoto Daimon¹  Noriko Ishigame¹  Kazunori Kageyama¹  Rie Desaki¹  Shinobu Takayasu¹  Shingo Murasawa¹  Yuki Nakada¹ 

[1] Department of Endocrinology and Metabolism, Hirosaki University Graduate School of Medicine, Aomori 036-8562, Japan;Health Care Center, Kochi University, Kochi 780-8520, Japan

关键词: Cushing’s disease;    Adrenocorticotropic hormone (ACTH);    Proopiomelanocortin (POMC);    Pituitary tumor;    Trichostatin;   

DOI  :  10.1507/endocrj.EJ15-0369

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(18)Cushing’s disease is primarily caused by adrenocorticotropic hormone (ACTH)-producing pituitary adenomas.Pituitary tumor-transforming gene 1 (PTTG1) expression, a hallmark of pituitary tumors, stimulates pituitary cell proliferation.Histone deacetylases (HDACs) play an important role in regulating gene transcription and HDAC inhibitors induce cellular differentiation and suppress tumor cell proliferation.HDAC inhibitors also repress PTTG1 mRNA levels.Trichostatin A (TSA) is a potent cell-permeable HDAC inhibitor that blocks cell cycle progression.In the present study, we determined the effect of TSA on ACTH production and cellular proliferation in mouse AtT-20 corticotroph tumor cells.TSA decreased proopiomelanocortin (POMC) mRNA levels in AtT-20 cells and reduced ACTH levels in the culture medium of these cells.The TSA-induced decreases in POMC mRNA levels were not modulated when TSA and dexamethasone were simultaneously administered.Drug treatment also decreased AtT-20 cell proliferation, induced apoptosis, and increased the percentage of cells in G0/G1 phase using flow cytometry.TSA decreased PTTG1 mRNA levels.Furthermore, PTTG1 knockdown inhibited cellular proliferation.Its knockdown also inhibited POMC mRNA and ACTH levels.TSA inhibits ACTH production and corticotroph tumor cell proliferation.TSA may inhibit cellular proliferation, and ACTH synthesis and secretion by decreasing PTTG1 expression.

【 授权许可】

Unknown