Nucleobindin-2 is a positive regulator for insulin-stimulated glucose transporter 4 translocation in fenofibrate treated E11 podocytes [Rapid Communication]

Endocrine Journal

Tetsurou Satoh²  Eijiro Yamada²  Jeffrey E. Pessin³  Yoko Shimoda²  Tsugumichi Saito²  Junichi Okada²  Yuko Tagaya²  Masatomo Mori⁴  Shuichi Okada²  Koshi Hashimoto¹  Masanobu Yamada² 

[1] Department of Metabolic Preemptive Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8510, Japan;Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan;Departments of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine, NY, USA;Kitakanto obesity and metabolic research institute, Midori 379-2311, Japan

关键词: Nucleobindin-2;    Podocyte;    Insulin resistance;    Glucose transporter 4 translocation;    Insulin;   

DOI  :  10.1507/endocrj.EJ14-0330

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(25)Cited-By(1)The physiology of insulin signaling under normal and disease conditions is well studied in classical insulin target tissues, but not in podocytes.To examine insulin stimulation of podocyte GLUT4 translocation, we established a protocol involving treatment with the PPARα agonist fenofibrate to induce E11 podocyte differentiation within 48 hours rather than 7-10 days, which is required for differentiation under the reported protocol.This allowed us to transiently introduce GLUT4 reporter cDNA and RNAi and thereby to examine the regulatory pathway involved.Here we demonstrate that treatment with 200μM fenofibrate for 36 hours following transfection had a dramatic effect on podocyte morphology, induced several podocyte specific protein expression markers (G protein-coupled receptor 137B, chloride intracellular channel 5, and nephrin) and resulted in insulin-stimulated GLUT4 translocation.In addition, Nucleobindin-2 was found to constitutively associate with Septin 7 (the repressor of GLUT4 translocation), and knockdown of Nucleobindin-2 was found to completely abrogate insulin-stimulated GLUT4 translocation.Together, these data suggest that Nucleobindin-2 may repress Septin7-induced inhibition of insulin-stimulated GLUT4 translocation in podocytes.

【 授权许可】

Unknown