Bacteria with antimicrobial properties isolated from the Mediterranean sponges Chondrilla nucula and Petrosia ficiformis

Aquatic Microbial Ecology

Elisabetta Chelossi¹  Raffaella Pantile Roberto Pronzato Martina Milanese Ute Hentschel¹ 

关键词: Antimicrobial activity;    Associated bacteria;    Chondrilla nucula;    Petrosia ficiformis;    Porifera;    16S rRNA;   

DOI  :  10.3354/ame01134

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Bacteria were isolated seasonally from the Mediterranean sponges Chondrilla nucula and Petrosia ficiformis and screened for antibacterial activities. Selected isolates were taxonomically identified by 16S rRNA gene sequencing. A total of 416 different bacterial strains were isolated, 60 (14.4%) of which displayed variable degrees of antimicrobial activity. Of the bioactive strains, 58.3% were able to inhibit Staphylococcus aureus, 6.7% were active against Bacillus subtilis, 11.7% against both Enterococcus faecalis and Escherichia coli, 38.3% against Pseudoalteromonas atlantica and 33.3% against Pseudomonas elongata. 16S rRNA gene sequence analysis showed that 2 isolates, 1 from seawater samples and 1 from P. ficiformis, were most closely related to Bacillus subtilis (99% similarity) and that another isolate from P. ficiformis was most closely related to a previously described sponge-associated Alphaproteobacterium NW001 (98% similarity). Two isolates from C. nucula were most closely related to Brachybacterium paraconglomeratum (99% similarity) and Shewanella algae (89% similarity). The high percentage of bioactive isolates derived from the 2 sponges suggests that marine microorganisms, whether animal-associated or planktonic, are promising sources for drug discovery.

【 授权许可】

Unknown