The Effect of Cytokines on Parathyroid Hormone-Related Protein (PTH-rP) Production in Human Amnion Cells

Endocrine Journal

YASUSHI SASAKI¹  TARO MORIMOTO¹  KIYTAKE ICHIZUKA¹  HIROSHI SAITO¹  MAKOTO SUZUKI¹  TAKUMI YANAIHARA¹  HITOMI KURIHARA¹ 

[1] Department of Obstetrics and Gynecology, Showa University School of Medicine

关键词: Parathyroid hormone-related protein;    mRNA;    Amnion cell;    Cytokine;   

DOI  :  10.1507/endocrj.46.479

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(31)Cited-By(2)In the present study, the effects of various cytokines on parathyroid hormone-related protein (PTH-rP) production and PTH-rP mRNA expression in human amnion cells were studied. Immunoreactive (ir) PTH-rP was measured by immunoradiometric assay and the expression of PTH-rP mRNA was determined by Northern blot analysis. The addition of interleukin-1β (IL-1β, 10ng/ml) and IL-6 (10ng/ml) in culture medium for 24 hours resulted in a significant increase in ir-PTH-rP levels by 1.5 and 1.6 fold, respectively. The effects of these agents were dose dependent. In contrast, IL-2 (10ng/ml) and IL-8 (10ng/ml) showed no effect on the production of ir-PTH-rP from amnion cells. Treatment with IL-1β or IL-6 for 6 hours increased the expression of PTH-rP mRNA in amnion cells. The stimulatory effect of IL-1β was reduced by IL-1 receptor antagonist (IL-1Ra) in a dose dependent manner. Both tetradecanoyl phorbol acetate (TPA), and forskolin increased PTH-rP mRNA levels and the PTH-rP production in amnion cells, and the effect of TPA was much greater than that of forskolin. The findings of the present study suggest of the participation of inflammatory cytokines for the regulation of PTH-rP production in human amnion cells.

【 授权许可】

Unknown