Development of a TaqMan PCR assay for the detection of Bonamia species

Diseases of Aquatic Organisms

Serge Corbeil¹  Isabelle Arzul Ben Diggles Mike Heasman Bruno Chollet Franck C. J. Berthe Mark St. J. Crane¹ 

关键词: Bonamia spp.;    Real-time TaqMan PCR assay;    Oyster;   

DOI  :  10.3354/dao071075

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The development of molecular diagnostic assays with increased sensitivity compared with conventional histological techniques is highly desirable for effective management of bonamiosis in cultured oyster stocks and wild populations. A real-time TaqMan PCR assay was developed for the specific detection of Bonamia species in infected oyster tissues. The TaqMan assay was shown to be significantly more sensitive than histopathology. Although a real-time TaqMan PCR assay is comparable with conventional PCR in terms of sensitivity, it offers the advantages that it is a rapid test and has a very low risk of sample cross-contamination. Furthermore, it can be optimised to quantify the parasite load in samples. The assay detected Bonamia isolates from Australia, New Zealand, Europe, Canada, Chile and the USA and therefore demonstrated genus specificity as tested in this study.

【 授权许可】

Unknown