Immune Recognition of Hormonogenic Sites of Human Thyroglobulin

Endocrine Journal

HIROSHI TAKUNO³  KENJI OKUDA⁴  HIROYUKI MAEKAWA³  IKUO MATSUI³  KIYOSHI MIURA³  SHIGEKI SAKATA³  OSAMU TARUTANI²  HIROSHI SARUI³  MASAFUMI MATSUDA³  TOMIO KOTANI¹  TORU OGAWA³ 

[1] Central Laboratory of Clinical Investigation, Miyazaki Medical College Hospital;Division of Biological Chemistry, Science University of Tokyo;The Third Department o f Internal Medicine, Gifu University School of Medicine;Department of Bacteriology, Yokohama-City University School of Medicine

关键词: Human thyroglobulin;    Antigenic site;    Synthetic peptides;    Anti-thyroid hormone antibodies;    Murine monoclonal antibody;   

DOI  :  10.1507/endocrj.40.393

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(22)Cited-By(1)We synthesized four peptides (HTg-1, 1-10; HTg-2, 2547-2558; HTg-4, 2592-2603 and HTg-6, 2737-2748) and two peptides (HTg-3, 2582-2591 and HTg-5, 2687-2694) with or without hormonogenic acceptor tyrosine of human thyroglobulin (hTg). They were iodinated with 127I or 125I. 127I-labeled peptides were tested for their ability to displace 125I-T4 binding to thyroid hormone autoantibodies (THAA) in two cases of Graves' disease and to a murine anti-hTg monoclonal antibody with anti-T4 activity (mAb). 125I-labeled peptides were tested for the direct binding to the aforementioned antibodies. None of the peptides displaced 125I-T4 binding to THAA or to a mAb, or exhibited increased binding to THAA and to a mAb. 125I-T4 binding to a mAb was equally displaced by hTgs obtained from a normal thyroid gland (NTg) and a case of Hürthle cell adenoma with undetectable iodine content (CTg). 125I-T4 binding to serum gamma globulin in each patient's serum was completely displaced by NTg, but CTg displaced 125I-T4 binding 2% and 5% in Case 1 and Case 2, respectively. It was speculated that the mAb recognizes a topological epitope around the hormonogenic site of hTg, while that of THAA in our two cases recognizes only T4 or an iodine dependent topological epitope(s) of hTg.

【 授权许可】

Unknown