Effects of Antenatal Dexamethasone on Antioxidant Enzymes and Nitric Oxide Synthase in the Rat Lung

Journal of Pharmacological Sciences

Koutaro Murano²  Yasushi Koitabashi²  Toshio Kumai¹  Yuko Takeba¹  Kentaro Asoh²  Takahiro Kojima²  Kenjiro Goto²  Shinichi Kobayashi¹  Masanori Mizuno²  Masaki Arima²  Yoshimitsu Tsuzuki¹ 

[1] Department of Pharmacology, St. Marianna University School of Medicine, Japan;Department of Pediatrics, St. Marianna University School of Medicine, Japan

关键词: reactive oxygen species;    antioxidant enzyme;    dexamethasone;    superoxide dismutase;    nitric oxide synthase;   

DOI  :  10.1254/jphs.FP0060844

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(29)Cited-By(9)We investigated the effects of prenatal dexamethasone (DEX) administration on antioxidant enzymes (AOEs) and nitric oxide synthase (NOS) in fetal and neonatal rat lungs. DEX (1 mg/kg, s.c., for 2 days) or vehicle alone was administered to pregnant rats, and the lungs of fetuses on days 19 and 21 of gestation and of 1- and 3-day-old neonates were examined. We measured protein levels of the AOEs manganese superoxide dismutase and copper-zinc superoxide dismutase (Mn SOD and Cu-Zn SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and inducible and endothelial nitric oxide synthase (i-NOS and e-NOS). Mn SOD, GSH-Px, and e-NOS expression gradually increased with increasing gestational and postnatal age in the lungs of the control groups. Cu-Zn SOD, CAT, and i-NOS expression did not change with increasing gestational and postnatal age in the lungs of the control groups. DEX administration had significant effects on i-NOS and e-NOS protein and mRNA expression. The increased Mn SOD, GSH-Px, and e-NOS expressions during the perinatal period suggests that antenatal developmental changes in AOEs in the lungs of premature fetuses could be reduced by reactive oxygen species–mediated injury at birth. Furthermore, antenatal glucocorticoid treatment may accelerate the development of lungs via the two types of NOS.

【 授权许可】

Unknown