Changes in the Localization of Type I, III and IV Collagen mRNAs in the Kidneys of Hereditary Nephritic (ICGN) Mice with Renal Fibrosis

Journal of Veterinary Medical Science

Noboru MANABE²  Misuzu YAMAGUCHI-YAMADA²  Kozue UCHIO²  Yoshie YAMAMOTO¹  Hajime MIYAMOTO²  Yasufumi GOTO²  Atsuo OGURA³ 

[1] Department of Veterinary Sciences, National Institute of Infectious Diseases;Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University;RIKEN Bioresource Center

关键词: extracellular matrix (ECM);    ICR-derived glomerulonephritis (ICGN) mouse;    in situ hybridization;    type I;    III and IV collagen mRNA;    type I;    III and IV collagen mRNA;   

DOI  :  10.1292/jvms.66.123

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(31)Cited-By(7)Renal fibrotic change, extreme accumulation of extracellular matrix (ECM) components in glomeruli and tubulointerstitum, is one of the characteristic features of ICR-derived glomerulonephritis (ICGN) mice. Decreased degradation of ECMs by matrixmetalloproteinases was demonstrated in kidneys of ICGN mice. To determine the balance between production and degradation of ECMs in kidneys of ICGN mice, we examined expression of mRNAs of ECMs in those. To demonstrate the localization of type I, III and IV collagen mRNAs in kidney sections of ICGN and control ICR mice, in situ hybridization using digoxigenin-labeled oligonucleotide antisense probes for procollagen-α1 (I), -α1 (III) and -α1 (IV) mRNAs, respectively, was performed. Negative or trace expressions of type I and III collagen mRNAs were observed in the kidneys of control mice, but stronger expressions of those were seen in glomeruli and injured renal tubules of the kidneys of ICGN mice. Moderate expression of type IV collagen mRNA was demonstrated in a part of glomeruli and renal tubules of both control and ICGN mice, and no remarkable difference was seen between them. Severe renal fibrosis, extreme accumulation of interstitial type I and III collagens is caused by increased production and decreased degradation in the kidneys of ICGN mice. Thus, the profiles of metabolism between interstitial and membranous collagens may be different in the kidneys of ICGN mice, and excessive production of interstitial collagens may be the dominant cause of renal disease in them.

【 授权许可】

Unknown