An Efficient Multiplex PCR Suitable for Large Scale Typing in Linkage Mapping

Journal of Veterinary Medical Science

Kozo MATSUMOTO¹  Takahisa YAMADA¹  Kaichun KWEI¹  Tomoe OGINO¹  Daniel H. MORALEJO¹  Suwen WEI¹ 

[1]Institute for Animal Experimentation, University of Tokushima School of Medicine, 3 Kuramoto, Tokushima 770-8503, Japan

关键词: gel loading dye;    linkage mapping;    multiplex PCR;   

DOI  :  10.1292/jvms.61.849

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)The dissection of polygenic traits is made possible with the development of microsatellite markers. Linkage study of this kind involves many markers with tens of hundreds of samples. Although typing essentially contains only two steps: PCR amplification and gel electrophoresis. Such work is still heavy when a large number of samples had to be genotyped. Multiplex PCR may reduce the work, but one has to optimize the conditions from marker to marker. Here we describe a dye-compatible multiplex PCR that works under standardized condition without the need to pre-determine the combinational primer concentration and the time-consuming step to mix many samples with gel loading dye before electrophoresis. This successful protocol should greatly reduce the cost and labor for genetic study of polygenic traits

【 授权许可】

Unknown