In vitro production of bovine embryos: utilization of different gonadotropin sources for in vitro maturation of oocytes

Revista Brasileira de Zootecnia

Coelho, Lia de Alencar³  Almeida Junior, Ivo Luis²  Esper, César Roberto²  Alvarez, Rafael Herrera¹  Vantini, Roberta² 

[1] Instituto de Zootecnia;UNESP;USP, Pirassununga

关键词: bovine;    cleavage rate;    embryo;    gonadotropin;    in vitro fertilization;    in vitro maturation    Introdução As condições de cultura in vitro para maturação dos oócitos são essenciais para a obtenção de resultados satisfatórios dentro do sistema de produção in vitro de embriões;   

DOI  :  10.1590/S1516-35982002000500007

学科分类：兽医学

来源: Universidade Federal de Vicosa

【 摘 要 】

- This study aimed to evaluate the effect of different gonadotropin sources for in vitro maturation of bovine oocytes fertilized and developed in vitro on cleavage (CR) and blastocysts rates (BLR). The immature oocytes from slaughterhouse cows ovaries were matured in vitro under different conditions: TCM 199 medium with 10% eostrous cow serum (ECS), aditives, hepes, NaHCO3, sodium pyruvate, antibiotics (B-199 medium), 20 UI/mL PMSG and 10 UI/ml hCG (PMSG/hCG) or B-199 medium with 5 mg/mL FSH and 5 mg/mL LH (FSH/LH). After 24 hours of culture at 38.5°C with 5% CO2 in air the mature oocytes were incubated with frozen-thawed semen for 18-21 hours. After this, the oocytes were transferred into microdroplets of Ménezo medium with 10% ECS and bovine oviduct ephitelial cells (BOEC) in suspension and were further cultured for 9 days. The data were analyzed by chi-square test. The CR and BLR for PMSG/hCG and FSH/LH were 60 and 13.9% and 61.2 and 10.6%, respectively. There was no difference between treatments regarding to CR and BLR. The results indicate that both gonadotropin sources can be used for in maturation medium of oocyte fertilized and developed in vitro.

【 授权许可】

CC BY-NC