Evaluating the effectiveness of different protocols for preparation of platelet rich plasma for use in equine medicine

Pesquisa Veterinária Brasileira

Alves, Ana Liz G¹  Yamada, Ana Lúcia M.¹  Carvalho, Armando de M¹  Maia, Leandro¹  Moraes, Lívia F.¹  Watanabe, Marcos J¹  Queiroz, Diana L¹  Vendruscolo, Cynthia P.¹ 

[1] Universidade Estadual Paulista, Botucatu, Brasil

关键词: Horse;    platelet-rich plasma;    centrifugation;    whole blood.;   

DOI  :  10.1590/S0100-736X2012000200002

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

Platelet-rich plasma (PRP) is a prepared of the whole blood which contains several growth factors responsible for cellular proliferation and differentiation, angiogenesis, and for the increase of the extracellular matrix. Thus, the aim of this study was to test 10 different centrifugation protocols to obtain PRP from the whole blood of healthy equines. Ten samples of 27mL of the whole blood of 5 healthy equines were used, which were centrifuged in accordance to the proposed protocols. The results showed that the protocols with less relative centrifugation force resulted in greater (p<0,05) platelets concentration. Also, platelets concentration was not influenced by varying the time of centrifugation. However, time did affect the number of leukocytes in some protocols. The best four protocols were analyzed by ELISA test to quantitate the amount of TGF-β, which revealed no difference (p> 0.05) between the protocols.INDEX

【 授权许可】

CC BY-NC