Screening of Multiple Potential Control Genes for use in Caste and Body Region Comparisons Using RT-qPCR in Coptotermes formosanus

Sociobiology

Dunhua Zhang⁴  Matthew R Tarver³  Christopher B Florane⁴  Christopher Mattison¹  Doug J Hinchliffe²  Alan R Lax⁴ 

[1] ARS-USDAFood Processing and Sensory Quality Research;ARS-USDACotton Chemistry and Utilization Research Unit;United States Department of Agriculture;ARS-USDAFormosan Subterranean Termite Research Unit

关键词: Solenopsis invicta;    Phenacoccus solenopsis;    honeydew;    preference;    foraging activity;   

DOI  :  

来源: Universidade Estadual de Feira de Santana, Brazil

【 摘 要 】

Formosan subterranean termites,Coptotermes formosanus , are a significant worldwide pest. Molecular gene expression is an important tool for understanding the physiology of organisms. The recent advancement of molecular tools forCoptotermes formosanusis leading to the advancement of the understanding of termite physiology. One of the first steps in analyzing gene expression is the normalization to constant reference genes. Stable reference genes that have constant expression across multiple treatments are important for accurately comparing target genes' expression. The objective of this investigation was to analyze and validate a set of potential reference genes including8S rRNA; Glyceraldehyde 3-phosphate dehydrogenase ( Gadphd ); ribosomal protein L7 ( RPL );β-actin (BA1) ;α-tubulin( Atube );α-actin( Aactin ); and elongation factor ( Elong ) as standards for analysis of transcriptional changes in the termiteCoptotermes formosanus , across two phenotypic castes, body regions, and colonies. We also compared the expression ofhexamerin -1 and 2 using stable and unstable reference genes to demonstrate the importance of consistent control genes. Our results demonstrate that18SandRPLcan serve as reliable expression standards when comparing these different castes and body regions, and we show thatC. formosanus Hex-1andHex-2have expression patterns similar to that previously described inR. flavipes .

【 授权许可】

Unknown