Association of Tumor-Associated Antigen on the Proliferation of Bovine Leukemia Virus-Infected Lymphoblastoid B-Cell Lines

Journal of Veterinary Medical Science

Atsuhiko HASEGAWA¹  Hajime TSUJIMOTO¹  Mhan-Pyo YANG¹  Misao ONUMA¹  Ryo GOITSUKA¹ 

关键词: bovine leukemia virus;    growth factor;    lymphoblastoid B cell;    tumor-associated antigen;   

DOI  :  10.1292/jvms.54.757

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)The association of tumor-associated antigen (TAA) on the proliferation of BLV-infected lymphoblastoid B-cell lines (BL2M3 and BL312) was investigated. Flow cytometric analysis of the expression of TAA with monoclonal antibody (mAb) c143 showed high expression of TAA on the surfaces of BL2M3 and BL312 cells. A large amount of TAA was found in the culture supernatant of BL2M3 and BL312 cells as well as in the lysates of BL2M3 and BL312 cells. Culture supernatant but not lysates of BL2M3 and BL312 cells promoted the growth of either BL2M3 cells or BL312 cells. Furthermore, this growth promoting activity in culture supernatants of BL2M3 and BL312 cells was inhibited in a dose-dependent manner when cultured with mAb c143. These results suggested that TAA may be involved in the growth factor-mediated cell growth of bovine B-lymphoblastoid cell lines expressing TAA on their cell surface.

【 授权许可】

Unknown