Isolation of Maedi/Visna Virus from a Sheep in Japan

Journal of Veterinary Medical Science

Jun SASAKI³  Atsushi KIMURA¹  Chiaki TANAKA²  Keisuke OGUMA²  Hiroshi SENTSUI²  Masanobu GORYO³  Ryo HARASAWA⁴ 

[1] Veterinary Clinics of NOSAI Morioka, 28�?2 Hosoda, Kamiohta, Morioka, Iwate 020�?0053, Japan;Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252�?0880, Japan;Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3�?18�?8 Ueda, Morioka, Iwate 020�?8550, Japan;Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3�?18�?8 Ueda, Morioka, Iwate 020�?8550, Japan

关键词: maedi/visna;    PCR;    retrovirus;    sheep;    virus disease;   

DOI  :  10.1292/jvms.13-0269

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(1)Maedi/visna (MV) is a lentiviral disease of sheep caused by the maedi/visna virus (MVV). Although MV is prevalent in many countries, it had not been reported in Japan. In 2011, however, three sheep in northern Japan were reported to be seropositive against the MVV antigen, indicating a persistent MVV infection. In the present study, we isolated MVV from one sheep to confirm MVV infection and conducted genomic classification of the virus. The co-culture of leukocytes from a seropositive sheep with fetal goat lung cells resulted in the formation of syncytial cells and the amplification of a long terminal repeat sequence of MVV by polymerase chain reaction. The isolate was confirmed as being MVV, rather than the caprine arthritis-encephalitis virus based on phylogenetic analysis of the gag gene sequence. Although the sheep was asymptomatic, nonpurulent meningitis and demyelination were found in the spinal cord. These were considered to be early lesions associated with pathogenic MVV infection. Therefore, the present study demonstrated that MVV is distributed in Japan.

【 授权许可】

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