Puritication and Identification of a Serum Protein Increased by Anthelmintic Drugs for Dirofilaria immitis in Dogs

Journal of Veterinary Medical Science

Bunei SYUTO¹  Masami MORIMATSU¹  Masayoshi MIIYAMA¹  Eiji UCHIDA¹  Masaki NAKAGAWA¹  Fuyu MIYOSHI¹  Masayuki SAITO¹  Noriko TOSA¹ 

关键词: acute phase protein;    anthelmintic;    Dirofilaria immitis;    dog;    haptoglobin;   

DOI  :  10.1292/jvms.55.27

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Cited-By(6)Polyacrylamide gel electrophoretic analysis of canine serum protein has revealed that the administration of anthelmintics elicits an increase in a certain serum protein. This protein, named PT60, was partially purified by ammonium sulfate fractionation and preparative electrophoresis. The purified PT60 gave a single band with the molecular size of 53 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under non-reducing conditions. After reduction with 2-mercaptoethanol, two bands appeared at 35 kDa and 17 kDa, indicating that PT60 consists of two subunits which are linked with each other by disulfide bonds. PT60 had the capacity to bind to hemoglobin. In an immunodiffusion test, an antiserum against PT60 cross-reacted with canine haptoglobin (Hp). N-terminal amino acid sequences of two PT60 subunits were identical to those of α and β subunits of canine Hp, respectively. Thus, PT60 was identified as Hp.

【 授权许可】

Unknown