Prophylactic treatment with sulphonated immunoglobulin G attenuatesdevelopment of mechanical allodynia-like response in mice with neuropathicpain

Journal of Veterinary Medical Science

Tsunefumi KOBAYASHI¹  Yasuhiro ITANO¹  Wataru YAMAMOTO¹  Yoshinori KASAHARA¹  Daishiro MIURA¹ 

[1] Pharmaceutical Development Research Laboratories, Teijin Institute for Bio-Medical Research, Teijin Pharma Limited, Tokyo 191�?8512, Japan

关键词: mechanical allodynia-like response;    neuropathic pain;    partial sciatic nerve ligation;    sulphonated immunoglobulin G;   

DOI  :  10.1292/jvms.15-0195

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(33)Human immunoglobulin G (IgG) concentrates are immune-modulating,anti-inflammatory plasma-derived products. Clinical studies in recent years have suggestedthat IgG attenuates neuropathic pain. In this study, effects of sulphonated IgG on thedevelopment and maintenance of a mechanical allodynia-like response were examined in micewith neuropathic pain induced by a partial sciatic nerve ligation (PSL). When sulphonatedIgG (400 or 1,000 mg/kg/day, i.p.) was administered for 5 days, from 1 day before surgeryto post-operative day (POD) 3, the development of a mechanical allodynia-like response wasattenuated. On the other hand, sulphonated IgG had little effect on the maintenance of amechanical allodynia-like response when administered for 5 days, from POD 11 to POD 15, atwhich time a mechanical allodynia-like response had already been developed. To explore themechanism of sulphonated IgG, the mRNA expression of inflammatory cytokines was evaluatedin the injured sciatic nerve. Sulphonated IgG (1,000 mg/kg/day, i.p.) that wasadministered for 3 days, from 1 day before surgery to POD 1, significantly attenuated theup-regulation of tumor necrosis factor-α and monocyte chemotactic protein-1 mRNAs on POD1. These results suggest that prophylactic treatment with sulphonated IgG attenuates thedevelopment of mechanical allodynia-like response by inhibition of inflammatory cytokineexpression in mice with PSL.

【 授权许可】

Unknown