Experimental horizontal transmission of viral hemorrhagic septicemia virus (VHSV) in Japanese flounder Paralichthys olivaceus

Diseases of Aquatic Organisms

H. Iida¹  M. Arimoto¹  K. Muroga¹  T. Nishizawa¹  K. Mori¹ 

关键词: Viral hemorrhagic septicemia virus;    Paralichthys olivaceus;    Horizontal transmission;    Immersion challenge;    Waterborne route;   

DOI  :  10.3354/dao058111

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Infection by viral hemorrhagic septicemia virus (VHSV) has recently occurred among wild and farmed Japanese flounder Paralichthys olivaceus in Japan. In the present study, horizontal transmission of VHSV among Japanese flounder wasexperimentally demonstrated by immersion challenge. Exposure to a flounder isolate (Obama25) of VHSV revealed a dose-response, with higher mortality (81 and 70%) at the 2 higher exposure levels (6.0 and 4.0 log₁₀ TCID₅₀ml^-1). In a second experiment, high titers of VHSV were expressed from moribund and dead flounder based on virus detection in holding-tank waters 2 to 3 d prior to death of the fish and 1 d after death. The virus could not be detected in tankwaters 2 d after death. Finally, a third cohabitation experiment in small tanks demonstrated horizontal transmission of VHSV from experimentally infected to uninfected fish.

【 授权许可】

Unknown