Characterization of Genes Encoding Virulence Determinants and Toxins in Staphylococcus aureus from Bovine Milk in Central Italy

Journal of Veterinary Medical Science

Fabrizio PASSAMONTI¹  Mauro COLETTI¹  Maria LUISA MARENZONI¹  Doreene R. HYATT²  Patrizia CASAGRANDE PROIETTI³  Giacomo COPPOLA¹  Annalisa BIETTA³ 

[1] Pathology, Diagnostic and Veterinary Clinic Department, Sport Horse Research Centre, University of Perugia;Department of Microbiology, Immunology, and Pathology, Colorado State University, U.S.A .(Hyatt),1619 Campus Delivery, Colorado State University;Department of Biopathological Science and Hygiene of Food and Animal Production, Faculty of Veterinary Medicine, University of Perugia

关键词: bovine;    PCR;    staphylococcal enterotoxins;    Staphylococcus aureus;    virulence determinants;   

DOI  :  10.1292/jvms.10-0158

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(39)Cited-By(11)The aim of this study was to evaluate the genotypic characteristics of Staphylococcus aureus isolates (n=170) from bovine milk collected from seven dairy farms in Italy. On the basis of cultural and biochemical properties and by amplification of the 23S rRNA specific to S. aureus, all isolates were identified as S. aureus. To genotypically characterize S. aureus isolates, genes encoding virulence determinants (nuc, clfA, spa-IgG-binding, spa-X-region, fnbA and fnbB, cap5 and cap8) and staphylococcal enterotoxins (sea, seb, sec, sed, see, seg, seh, sei, sej) were investigated using a PCR technique. The results showed that the isolates of S. aureus in each farm had the same genotypic characteristics, while the isolates genotipically differed between the different farms. The present study might help to understand the distribution of prevalent S. aureus strains in dairy farms.

【 授权许可】

Unknown