Influence of temperature on Ranavirus infection in larval salamanders Ambystoma tigrinum

Diseases of Aquatic Organisms

Kathryn Richards¹  Elizabeth W. Davidson¹  Santos Rojas¹  James K. Jancovich¹ 

关键词: Epizootic;    Cell culture;    Iridovirus;    Mortality;   

DOI  :  10.3354/dao063095

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT:Temperature strongly influenced percent mortality and time to death of salamanders exposed to the Ambystoma tigrinum virus (iridovirus) (ATV). Most salamanders survived when exposed at 26°C, whereas all died at 18°C and nearlyall died at 10°C. Some asymptomatic salamanders that survived 60 d at 10 or 26°C were found to be carrying virus. Polymerase chain reaction (PCR) confirmed the presence of virus in ATV-exposed salamanders but was found to be less sensitive thancell culture in detecting ATV at low concentrations. PCR products were 100% identical to ATV in the major capsid protein sequence. Virus titer was higher in salamanders held at 10°C than at 18°C but little virus, if any, was present in the smallnumber of salamanders that died at 26°C. These results may help explain periodic viral epizootics in field populations of A. tigrinum where water temperatures fluctuate widely.

【 授权许可】

Unknown