Sex Determination of Sika Deer (Cervus nippon yesoensis) Using Nested PCR from Feces Collected in the Field

Journal of Veterinary Medical Science

Masatsugu SUZUKI⁴  Yuta MOTOI²  Makoto ASANO⁴  Shoki YAMAZAKI²  Kazuya NAGAI¹  Tsuyoshi ISHINAZAKA³ 

[1] Faculty of Agriculture, Iwate University;The United Graduate School of Veterinary Sciences, Gifu University;Shiretoko Nature Foundation;Faculty of Applied Biological Sciences, Gifu University

关键词: feces;    nested PCR;    noninvasive;    sex determination;    sika deer;   

DOI  :  10.1292/jvms.11-0235

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(32)Cited-By(2)We describe a method for determining the sex of sika deer (Cervus nippon yesoensis) from feces collected in the field. Using a nested polymerase chain reaction (nested PCR), partial sequences of the sex determination region of the Y chromosome (SRY) gene and X zinc finger protein (ZFX) gene were amplified. In 19 individuals with sex information, the correct sex was successfully detected and sequences of target amplicons were completely matched between muscle and feces from the rectum. Among 75 fecal samples collected noninvasively in the field, 68-71 samples (90.7-94.7%) yielded successful sex determinations. Using this technique, feces collected in the field would enhance the utility of genetic analysis. As a result, instead of biomaterials, these samples can serve as new or alternative materials. Finally, it can be expected that this technique will contribute to reveal in advanced detail of the population dynamics and genetic diversity that needed to carry out effective population control and to reduce the extinction risk of sika deer.

【 授权许可】

Unknown