Detection of Lawsonia intracellularis Using Immunomagnetic Beads and ATP Bioluminescence

Journal of Veterinary Medical Science

Yosuke YAMATO¹  Suk KIM¹  Hidefumi FURUOKA³  Kayo MURAKATA³  Masahisa WATARAI¹  Yoshitaka OMATA² 

[1] Department of Applied Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine;Department of Basic Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine;Department of Pathological Science, Obihiro University of Agriculture and Veterinary Medicine

关键词: immunomagnetic beads;    Lawsonia intracellularis;    rabbit;   

DOI  :  10.1292/jvms.67.449

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(13)Cited-By(4)Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in various animals. The detection of L. intracellularis in clinical and environmental samples is necessary for the diagnosis of infection and epidemiological investigations. For the detection of L. intracellularis in fecal samples, we have developed an immunological method using immunomagnetic separation and ATP bioluminescence. Magnetic beads were coated with an anti-Lawsonia surface antigen (LsaA) antibody in order to capture the L. intracellularis in fecal samples from infected rabbits and the bacteria captured by the immunomagnetic beads were assayed by means of ATP bioluminescence. Our results showed that L. intracelluraris was detected by immunomagnetic separation of bacteria-holding magnetic beads and ATP-based bioluminescence, suggesting that our methods could be useful for the diagnosis of proliferative enteropathy.

【 授权许可】

Unknown