Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

Ciência Rural

Eiras, Marcelo²  Nascimento, Monique Bezerra¹  Fajardo, Thor Vinícius Martins³  Nickel, Osmar³  Pio-Ribeiro, Gilvan¹ 

[1] Universidade Federal Rural de Pernambuco, Recife, Brazil;Instituto Biológico, São Paulo, Brasil;Embrapa Uva e Vinho, Bento Gonçalves, Brazil

关键词: diagnosis;    sequencing;    Ilarvirus;    PNRSV;    ApMV;    ArMV;   

DOI  :  10.1590/0103-8478cr20141810

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

:There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

【 授权许可】

Unknown