Journal of Pharmacological Sciences | |
Evidence for M2 and M3 Muscarinic Receptor Involvement in Cholinergic Excitatory Junction Potentials Through Synergistic Activation of Cation Channels in the Longitudinal Muscle of Mouse Ileum | |
Hayato Matsuyama2  Masahisa Yamada4  Yasuyuki Tanahashi1  Toshihiro Unno2  Seiichi Komori2  Takio Kitazawa3  | |
[1] Laboratory of Pharmacology, Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Japan;Laboratory of Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University, Japan;Department of Pharmacology, School of Veterinary Medicine, Rakuno Gakuen University, Japan;Common Resources Group, Okinawa Institute of Science and Technology, Japan | |
关键词: muscarinic receptor; smooth muscle; nonselective cation channel; excitatory junction potential (EJP); small intestine; | |
DOI : 10.1254/jphs.12231FP | |
学科分类:药学 | |
来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society | |
【 摘 要 】
References(37)Cited-By(3)Cholinergic nerve–mediated excitatory junction potentials (EJPs) in the longitudinal muscle of mouse ileum were characterized by using M2 or M3 muscarinic receptor–knockout (KO) mice and 1-[β-[3-(4-methoxyphenyl) propoxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride (SK&F 96365) and pertussis toxin (PTX). EJPs evoked by electrical field stimulation (EFS) in wild-type preparations, initially determined to be cholinergic in origin using tetrodotoxin, atropine, and eserine, were profoundly depressed after SK&F 96365 treatment known to block muscarinic receptor–operated cation channels. A similar depression of the EJPs was also observed by PTX treatment, which is predicted to disrupt M2-mediated pathways linked to cation channel activation. In M2-KO mouse preparations, cholinergic EJPs were evoked by EFS with their relative amplitude of 20% – 30% to the wild-type EJP and strongly inhibited by SK&F 96365. No cholinergic EJP was seen in M3-KO as well as M2/M3 double-KO preparations. The results suggest that the wild-type cholinergic EJP is not a simple mixture of M2 and M3 responses, but due to synergistic activation of cation channels by both M2 and M3 receptors in the murine ileal longitudinal muscle.
【 授权许可】
Unknown
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