Cross-Reactivity of Japanese Encephalitis Virus-Vaccinated Horse Sera in Serodiagnosis of West Nile Virus

Journal of Veterinary Medical Science

Hideki NISHI⁴  Shinya SHIMIZ²  Jiro HIROTA²  Hiroshi TSUNEMITSU¹  Hiroshi MATSUDA³ 

[1] Research Team for Viral Diseases, National Institute of Animal Health, National Institute of Animal Health, National Agriculture and Food Research Organization;Research Team for Advanced Biologicals, National Institute of Animal Health, National Agriculture and Food Research Organization;Laboratory of Clinical Immunology, Department of Veterinary Clinic, Faculty of Agriculture, Tokyo University of Agriculture and Technology;Hokkaido Hidaka Livestock Hygiene Service Center

关键词: arthropod-borne viruses;    diagnosis;    equine;    Flavivirus;    horse;   

DOI  :  10.1292/jvms.09-0311

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(14)Flavivirus-infected sera are known to show cross-reactions in serodiagnoses of heterologous flavivirus infections. Japanese encephalitis virus (JEV) is endemic in Asia and, in Japan, many horses are vaccinated against JEV. However, the cross-reactivity level of JEV-vaccinated horse sera in the serodiagnosis of West Nile virus (WNV) has not been clarified. The antibody cross-reactivity of JEV-vaccinated horse sera in WNV serological tests, such as the plaque reduction neutralization test (PRNT), IgG indirect ELISA (IgG-ELISA) and hemagglutination inhibition (HI) test, was examined. All JEV-vaccinated horse sera were positive for JEV antibodies with JEV PRNT at both 90% and 50% plaque reductions. In WNV PRNT, 16.7% of the horses were positive at 90% plaque reduction, and 50% of the horses were positive at 50% plaque reduction. All the JEV-vaccinated horse sera showed positive-to-negative (P/N) ratios of over 2.0 with JEV IgG-ELISA, and half of them had P/N ratios of over 2.0 with WNV IgG-ELISA. There was little difference between the JEV HI and WNV HI titers in individual horses. These results indicate that in serosurveillance of WNV, JEV-vaccinated horses can produce false-positive results in WNV IgG-ELISA, HI and PRNT.

【 授权许可】

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