Compensatory Renal Growth in Uninephrectomized Immature Rats: Proliferative Activity and Epidermal Growth Factor

Journal of Veterinary Medical Science

Masaki MINO²  Keiko KATO²  Mika OMOTO-KITAO²  Tomohiro KONDO²  Masafumi MUKAMOTO³  Ken Takeshi KUSAKABE²  Toshiya OKADA²  Jun NAKAMURA¹  Ai TAKESHITA² 

[1] Department of Environmental Sciences and Engineering, University of North Carolina;Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University;Department of Infectious Diseases Control, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University

关键词: EGF;    glomerulus;    PCNA;    proximal tubules;    uninephrectomy;   

DOI  :  10.1292/jvms.09-0496

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(30)Cited-By(4)Compensatory response to uninephrectomy in immature animals is stronger compared with that in adult ones and the response is due mainly to renal cell proliferation. The present study explored to show the growth pattern of the remaining kidney immediately after uninephrectomy in immature rats with special reference to proliferating activity and epidermal growth factor (EGF). Immunolocalizations of proliferating cell nuclear antigen (PCNA) and EGF in immature rat kidney were examined during the first three days after uninephrectomy. Semi-quantitative analysis of the expression of preproEGF mRNA was performed. One day after the operation, the PCNA positive cell ratios in the glomeruli and the proximal tubules were significantly higher in unilaterally nephrectomized (UNx) rats than in sham-operated (Sham) rats. In UNx and Sham rats, the proximal and distal tubular cells showed positive reactions to EGF antibody. The positive reaction of proximal tubules to EGF antibody was weaker in UNx than in Sham rats 1 day after the operation, while the degree of reactivity was not different between UNx and Sham rats 3 days after the operation. The level of expression of preproEGF mRNA in the kidney was significantly lower in UNx than in Sham rats 1 day after the operation. These results indicate that unilateral nephrectomy in immature rats causes increased proliferative activity and decreased expression of EGF in the remaining kidney during the early period of compensatory renal growth.

【 授权许可】

Unknown