DIAGNOSIS OF Anaplasma marginale USING BLOOD CLOT FOR THE DETECTION OF PARASITEMIA

Ciência Rural

Araújo, Flávio Antônio Pacheco de¹  Stobbe, Neusa Saltiél¹  Chaplin, Eunice Leonora¹  Paiva, Maria de Graça de Souza¹  Fortes, Elinor²  Silva, Nilton Rogério Santos da¹ 

[1] Faculdade de Veterinária;Instituto de Biociências

关键词: blood smear;    clot smear;    clot impression smear;    hematozoan;    giemsa;   

DOI  :  10.1590/S0103-84781991000300008

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

A 6-month-old splenectomized calf was inoculated with blood from a cattle with Tick Fever. The clinical signs, rectal temperature and packed cell volume were observed between the 0 and 40th days. Blood and blood clot samples were collected from the calf on the 8th, 10th, 22th, 24th and 26th days. The following techniques were evaluated by the Anaplasma marginale parasitemia: thin blood smear of blood clot fragment and smear of a drop of blood clot. The slides were stained by the dilute and pure Giemsa methods. The results were acceptable and the authors propose the use of the three techniques to confirm an Anaplasma diagnosis.

【 授权许可】

CC BY-NC