High Genetic Diversity of Anaplasma marginale Detected fromPhilippine Cattle

Journal of Veterinary Medical Science

Adrian Patalinghug YBAÑEZ³  Xuen XUENAN¹  Mary Jane CRUZ-FLORES⁵  Florencia G. CLAVERIA⁵  Naoaki YOKOYAMA²  Rochelle Haidee D. YBAÑEZ⁴  Hisashi INOKUMA³ 

[1] National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080�?8555, Japan;United Graduate School of Veterinary Sciences, Gifu University, Gifu, Gifu 501�?1193, Japan;Department of Clinical Veterinary Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080�?8555, Japan;Visayas State University, Visca, Baybay City 6521-A, Leyte, Philippines;Biology Department, College of Science, De La Salle University-Manila, Taft Avenue, Manila 1000, Philippines

关键词: Anaplasma marginale;    cattle;    groEL;    Msp1a;    Philippines;   

DOI  :  10.1292/jvms.13-0405

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(34)Cited-By(4)A total of 658 cattle in 6 provinces in the Philippines were screened forAnaplasma marginale infection by using a diagnostic heat-shock operon(groEL) gene-PCR assay. The screening-positive samples were furthertested using the major surface antigen protein 1a (Msp1a) gene-PCR assay.Screening PCR results showed 130 cattle (19.8%) were positive for the A.marginale infection. Subsequent amplification using the Msp1agene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeatstructures, including 20 novel structures, and 41 distinct genotypes were identified.Interestingly, multiple infections of 4 different genotypes were also observed inA. marginale-infected cattle. The present study demonstrated theprevalence and characterization of diverse genotypes of A. marginale inthe Philippine cattle.

【 授权许可】

Unknown