The Complete Amino Acid Sequence of Dog β2-Microglobulin

Journal of Veterinary Medical Science

Fumio Hoshi¹  Yuichi Nakajima¹  Seiichi Higuchi¹  Seiichi Kawamura¹ 

[1]Laboratory of Veterinary Internal Medicine, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan

关键词: amino acid sequence;    dog β2-microglobulin;    purification;    urine;   

DOI  :  10.1292/jvms.61.517

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(29)Cited-By(3)Dog β2-microglobulin was purified from the urine of dogs with potassium dichromate induced tubular damage. It was purified by sequential use of anion exchange chromatography, gel filtration chromatography, and reversed-phase high performance liquid chromatography. Comparisons of the amino acid sequence of the dog protein with human, mouse, and rabbit β2-microglobulin, indicated a high degree of similarity. The dog protein was very similar to human β2-microglobulin in that it had a molecular weight of 11.8 kDa and contained two half-cystinyl residues. Dog and human β2-microglobulin were demonstrably different at 24 of the 99 positions compared. The data supported the conclusion that the purified protein was dog β2-microglobulin and that all four proteins from dog, human, mouse, and rabbit were closely related.

【 授权许可】

Unknown