Histopathological Studies on Cases of Chronic Mouse Hepatitis by Natural Helicobacter Infections

Journal of Veterinary Medical Science

Toshiaki KOKUBO¹  Kazuyuki UCHIDA³  Tetsu NISHIKAWA¹  Chunja NAM²  Hiroyuki NAKAYAMA³  Yasushi OHMACHI² 

[1] Laboratory Animal Section, Department of Technical Support and Development, Fundamental Technology Center, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba, 263–8555, Japan;Animal Pathology Section, Department of Advanced Technologies for Radiation Protection Research, Research Center for Radiation Protection, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba, 263–8555, Japan;Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo, 113–8657, Japan

关键词: C3H/HeNrs mouse;    chronic hepatitis;    Helicobacter;    natural infection;   

DOI  :  10.1292/jvms.12-0304

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(1)It is known that Helicobacter hepaticus or Helicobacter bilis infection causes chronic inflammation of the colon and liver. Chronic active hepatitis was found in radiation exposure experiments using male C3H/HeNrs mice at our institute. Histopathologically, 103 cases among 978 mice (64–91 weeks of age at autopsy) had hepatic lesions regardless of irradiation exposure. Mild lesions showed only focal necrosis and focal inflammation in the liver. Severe cases were accompanied by hepatocytomegaly, bile duct hyperplasia, hypertrophy and activation of Kupffer cells, cholangitis, pleomorphic hepatocytes and/or tumor. Helical-shaped bacteria were detected between hepatocytes by Warthin-Starry silver stain and immunohistochemistry (IHC) with an antibody against Helicobacter pylori. It was suggested that these cases of chronic hepatitis were caused by Helicobacter spp. Although chronic hepatitis occurred frequently in mice exposed high-dose irradiation compared with nonirradiated mice in one lot, it was not concluded that radiation might influence the incidence or degree of hepatitis. Our report suggested that natural Helicobacter spp. infection in mice can occur in an experimental animal facility. Therefore, it is suggested that monitoring of Helicobacter infection is very important for quality control of animal experiments.

【 授权许可】

Unknown