Measurement of Erythrocyte Carbonic Anhydrase Isozymes (CA-I and CA-II) in Racehorses and Riding Horses

Journal of Veterinary Medical Science

Nobutsune ICHIHARA³  Masako TAKAHASI³  Masao ASARI³  Tamae KASUYA²  Kumi MATSUI²  Toshiho NISHITA²  Masaru MURAKAMI¹ 

[1] Laboratory of Molecular Biology, School of Veterinary Medicine, Azabu University;Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University;Laboratory of Veterinary Anatomy 1, School of Veterinary Medicine, Azabu University

关键词: carbonic anhydrase;    equine;    ELISA;    erythrocyte;   

DOI  :  10.1292/jvms.67.63

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(4)Equine carbonic anhydrase isozymes (CA-I and CA-II) were purified from erythrocytes by several column chromatography. Polyclonal anti-CA-I and anti-CA-II sera were produced in rabbits. Sensitive competitive enzyme-linked immunosorbent assays (ELISA) were established to determine the developmental changes in CA-I and CA-II levels in equine erythrocytes. Concentrations of CA-I and CA-II in erythrocytes from 150 clinically normal thoroughbreds (123 racehorses and 27 riding horses) were determined by ELISA. Mean (± SD) concentrations of CA-I and CA-II in racehorses were 1.70 ± 0.48 and 0.94 ± 0.13 mg/g hemoglobin (Hb), respectively. Mean concentrations of CA-I and CA-II in riding horses were 2.34 ± 0.52 and 0.76 ± 0.08 mg/g Hb, respectively. When the CA levels in racehorses and riding horses were compared, the CA-I level in riding horses was higher than that in racehorses (p=0.01). The CA-II level in racehorses was higher than that in riding horses (p=0.02). These data suggest that the levels of CA isozymes in erythrocytes of racehorses were influenced by chronic physical stress. The CA-I concentration in erythrocytes of 2-month-old horses was approximately 0.25 mg/g Hb. The CA-I level noticeably increased during the first year of life and approached normal adult levels by 2 years. The CA-II level decreased slightly with age, indicating different regulation of CA-I and CA-II expression during development.

【 授权许可】

Unknown