Molecular Cloning of Canine Thrombomodulin cDNA and Expression in Normal Tissues

Journal of Veterinary Medical Science

Atsuhiko HASEGAWA³  Mikihiko TOKURIKI²  Hajime TSUJIMOTO¹  Toshihiro WATARI²  Haruhiko MARUYAMA²  Keisuke OGUMA³  Rui KANO³  Sadatoshi MAEDA¹ 

[1] Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Laboratory of Comprehensive Veterinary Clinical Studies, Department of Veterinary Medicine, Nihon University;Laboratory of Veterinary Pathobiology, Department of Veterinary Medicine, Nihon University

关键词: canine;    cDNA cloning;    thrombomodulin;   

DOI  :  10.1292/jvms.66.1423

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Thrombomodulin (TM) is a glycoprotein localized mainly on endothelial cell surfaces, and is a major regulator of vascular thromboresistance. The entire open reading frame of canine TM cDNA comprises 1737 bp, encoding 578 amino acid residues. Comparison of the deduced amino acid sequence from canine TM with those of human, mouse, rat, rabbit and bovine (partial) TM sequences revealed 73.1%, 69.1%, 65.8%, 74.3% and 69.5% identity, respectively. Canine TM mRNA expression was confirmed by RT-PCR analysis in lung, liver, spleen, kidney, pancreas and lymph node, and was relatively low in heart, cerebrum, urinary bladder and uterus. The present results provide valuable data for research into canine coagulation disorders.

【 授权许可】

Unknown