Cellular Ca2+ Dynamics in Urinary Bladder Smooth Muscle From Transgenic Mice Overexpressing Na+-Ca2+ Exchanger

Journal of Pharmacological Sciences

Susumu Ohya¹  Hidemichi Murata¹  Shingo Hotta¹  Takahiro Iwamoto²  Yuji Imaizumi¹  Hisao Yamamura¹  Eiji Sawada¹  Satomi Kita² 

[1] Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan;Department of Pharmacology, School of Medicine, Fukuoka University, Japan

关键词: Na+-Ca2+ exchanger;    urinary bladder smooth muscle;    transgenic mouse;   

DOI  :  10.1254/jphs.09319SC

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(2)The rise of Ca2+ concentration ([Ca2+]i) by reducing external Na+ in urinary bladder smooth muscle cells (UBSMCs) from transgenic mice overexpressing Na+/Ca2+ exchanger type-1.3 (NCX1.3tg/tg) was about 4 times as large as that in the wild-type (WT). NCX1 protein expression in UB increased about 4-fold in NCX1.3tg/tg. The Ca2+ release by caffeine in UBSMCs was comparable between NCX1.3tg/tg and WT, but [Ca2+]i decay was faster in NCX1.3tg/tg. Contractions induced by acetylcholine, 60 mM K+, or electrical stimulation were significantly smaller in UB segments of NCX1.3tg/tg. NCX worked in Ca2+-extrusion mode during these contractions in UBSMCs of both WT and NCX1.3tg/tg.

【 授权许可】

Unknown