Elevated temperature treatment as a novel method for decreasing p57 on the cell surface of Renibacterium salmoninarum

Diseases of Aquatic Organisms

Jon D. Piganelli¹  ,, Gregory D. Wiens¹  ,, Stephen L. Kaattari¹ 

关键词: Renibacterium salmoninarum;    Bacterial kidney disease;    p57;   

DOI  :  10.3354/dao036029

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Renibacterium salmoninarum is a Gram-positive diplo-bacillus and the causative agent of bacterial kidney disease, a prevalent disease of salmonid fish. Virulent isolates of R. salmoninarum have a hydrophobic cellsurface and express the 57-58 kDa protein (p57). Here we have investigated parameters which effect cell hydrophobicity and p57 degradation. Incubation of R. salmoninarum cells at 37°C for >4 h decreased cell surface hydrophobicity asmeasured by the salt aggregation assay, and decreased the amount of cell associated p57. Incubation of cells at lower temperatures (22, 17, 4 or -20°C) for up to 16 h did not reduce hydrophobicity or the amount of cell associated p57. Both the loss ofcell surface hydrophobicity and the degradation of p57 were inhibited by pre-incubation with the serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF). Cell surface hydrophobicity was specifically reconstituted by incubation with extracellularprotein (ECP) concentrated from culture supernatant and was correlated with the reassociation of p57 onto the bacterial cell surface as determined by western blot and total protein stain analyses. The ability of p57 to reassociate suggests that thebacterial cell surface is not irreversibly modified by the 37°C treatment and that p57 contributes to the hydrophobic nature of R. salmoninarum. In summary, we describe parameters effecting the removal of the p57 virulence factor and suggestthe utility of this modification for generating a whole cell vaccine against bacterial kidney disease.

【 授权许可】

Unknown