Effects of Lipopolysaccharide on Production of Interleukin-1 and Interleukin-6 by Bovine Mammary Epithelial Cells in vitro

Journal of Veterinary Medical Science

Rikio KIRISAWA⁴  Tomo-p YOSHINO²  Hiromichi OHTSUKA⁵  Thomas J. Thomas J. ROSOL³  Yuich YOKOMIZO¹  Satoru KON-NAI²  Hiroyuki OKADA² 

[1] National Institute of Animal Health, 3-1-1 Kan-nondai, Tsukuba, Ibaraki 305-0856, Japan;Department of Veterinary Pathology, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan;Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210-1093, U.S.A.;)Veterinary Microbiology, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan;Veterinary Teaching Hospital, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan

关键词: cytokine;    lipopolysaccharide;    mammary;   

DOI  :  10.1292/jvms.61.33

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Cited-By(17)This investigation was performed to determine the effect of lipopolysaccharide (LPS) on production of interleukin (IL)-1 and IL-6 by bovine mammary epithelial cells in vitro. After confluence, the cells were stimulated with LPS (0.1, 1.0 or 10 μg/ml) for 4, 8, 24, and 48 hr. LPS increased production of both IL-1 and IL-6 production from mammary cells in a dose dependent manner. The expression of mRNA for IL-1 receptor antagonist (IL-1ra) was demonstrated by reverse transcription-polymerase chain reaction in bovine mammary epithelial cells.

【 授权许可】

Unknown