False Positive Responses of Campylobacter jejuni when Using the Chemical-In-Plug Chemotaxis Assay

Journal of Veterinary Medical Science

Shinji TAKAI¹  Tsutomu KAKUDA¹  Doungjit KANUNGPEAN¹ 

[1]Laboratory of Animal Hygiene, School of Veterinary Medicine, Kitasato University

关键词: Campylobacter jejuni;    chemical-in-plug assay;    chemotaxis;   

DOI  :  10.1292/jvms.10-0396

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(13)Cited-By(3)Campylobacter jejuni reportedly exhibits chemotactic behavior towards fucose, several amino acids and organic acids, mucin and bile. The chemotaxis of C. jejuni has mainly been studied using the chemical-in-plug chemotaxis assay. In this study, a nonchemotactic mutant (cheY mutant) and nonmotile mutant (flhA mutant) were constructed and used as negative controls in an assay. Apparent zones of accumulation around test plugs containing several amino acids and organic acids were observed with both of the mutants. Our results suggest that positive responses of C. jejuni in the chemical-in-plug assay are not always indicative of chemotaxis.

【 授权许可】

Unknown