Identification of human papillomavirus in patients with anal squamous cell carcinoma and its relation with the grade of cellular differentiation and staging

Revista Brasileira de Coloproctologia

Matos, Delcio²  Villa, Luisa Lina³  Ferreira, Silvaneide¹  Soares, Paulo Cardoso⁴ 

[1] Instituto Ludwig de Pesquisa sobre o Câncer, São Paulo, Brasil;Escola Paulista de Medicina Universidade Federal de São Paulo, São Paulo, Brasil;Instituto Ludwig de Pesquisa sobre o Câncer;Hospital Ophir Loyola

关键词: papillomavirus infections;    carcinoma;    squamous cells;    anal canal;    papilloma.;   

DOI  :  10.1590/S0101-98802011000100002

学科分类：心脏病和心血管学

来源: Sociedade Brasileira de Coloproctologia

【 摘 要 】

OBJECTIVE:To identify the most predominant types of human papillomavirus (HPV) in carriersof the anal canal carcinoma (ACC), relating them to the cell differentiation andlesion staging degree, in patients from Belém, Pará, Brazil, between1998 and 2000.
METHODS: A case-control study was conducted with 75patients, divided into Test Group, with 33 carriers of the anal canal carcinoma,and Control Group, with 42 carriers of non-neoplastic diseases of the anal canal.The methods employed to identify the viral types were the polymerase chain reactionand the dot blot. The Fisher's exact test was used to assess the HPV occurrence.The distribution of HPV types was analyzed by 3x2 contingency tables, representingthe distribution of HPV types. For hypothesis testing, the significance levelα=0.05was previously established for rejection of the null hypothesis.
RESULTS:HPV was significantly prevalent (p=0.0027) in the Test (60.6%) and Control Groups(26.2%) (p=0.0027). The most prevalent viral types were HPV 16 (42.4%) and 18(15.2%). Significant differences related to the prevalence of HPV 16 and 18 wereverified between both groups (p=0.027 and p=0.043, respectively). In the ControlGroup, HPV 16 was found in 19.0%, whereas HPV 18 was observed in 2.4%. In theTest Group, we evaluated the distribution of HPV types according to the stagingand degree of cell differentiation, and found no significant differences betweenthe results of the different groups.
CONCLUSION: Squamous cell carcinomaof the anal canal is associated with the HPV presence, and the 16 and 18 typesare the most frequently found.

【 授权许可】

CC BY-NC