Cuban strategy for the molecular characterization of the pandemic influenza A virus (H1N1)

Revista Cubana de Medicina Tropical

Goyenechea Hernández, Ángel¹  Quilarte García, Elías¹  Hernández Espinosa, Bárbara¹  Llop Hernández, Alina¹  Oropesa Fernández, Suset Isabel¹  Piñón Ramos, Alexander¹  Valdés Ramírez, Odalys¹  González Muñoz, Grehete¹  Guzmán Tirado, María Guadalupe¹  Arencibia García, Amely¹  Kourí Cardellá, Vivian¹  Acosta Herrera, Belsy¹  González Baez, Guelsys¹  Savón Valdés, Clara Estela¹ 

[1] Instituto de Medicina Tropical Pedro Kourí, La Habana, Cuba

关键词: : pandemic influenza;    sequencing;    molecular characterization;    Cuba;   

DOI  :  

学科分类：传染病学

来源: SciELO

【 摘 要 】

INTRODUCTION: in April 2009, there was identified a variant of the A/H1N1 influenza virus of swine origin, and shortly after the first pandemic in XXI century was declared. OBJECTIVES: to establish a nucleotide sequencing strategy for the differential diagnosis of the seasonal and pandemic influenza A viruses, and to obtain as much molecular information as possible about hemagglutinin and neuraminidase genes in patients with influenza-like illnesses, in those with severe respiratory infection and in patients who died. METHODS: three sequencing strategies were designed and implemented, which also offered important information about the new virus in Cuba. RESULTS: the third strategy provided the most comprehensive results such as differential diagnosis, the surveillance of the D222G/E mutation in hemagglutinin and Tamiflu-resistant H275Y viral variants. In spite of the fact that the mentioned mutations were not detected, their presence in the Cuban population can not be ignored since these strategies were not designed for this end. It is imperative to design a study to fulfill this objective. CONCLUSIONS: the sequencing strategies in our algorithm allowed the differential diagnosis of the seasonal and the pandemic viruses, and their molecular characterization.

【 授权许可】

CC BY-NC