Spontaneous Differentiation of Adult Rat Marrow Stromal Cells in a Long-Term Culture

Journal of Veterinary Medical Science

Chun-Jung CHEN¹  Ching-Chung SHEU²  Pang-Yen TSENG²  Yong-San HUANG²  Chen-Wei YU² 

[1] Department of Education and Research, Taichung Veterans General Hospital;Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University

关键词: growth factor;    long-term culture;    marrow stromal cell;    neural differentiation;   

DOI  :  10.1292/jvms.69.95

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(35)Cited-By(19)It is well recognized that bone marrow stromal cells (MSCs) can differentiate into neuron-like cells when supplemented with growth factors and/or chemical treatments. We demonstrated that primary MSCs obtained from adult rats could spontaneously differentiate into neural precursor cells after long-term culture. During the outset of in vitro culture, less than 0.1% of adult rat primary MSCs expressed nestin, the common protein of neural precursors. These MSCs didn't show neuronal morphology nor express neuronal antigens. In contrast, after continuous maintenance for 6 weeks, a significant subpopulation of MSCs formed cellular clumps and expressed nestin (32.3 ± 6.3%). Less than 0.1% of cells expressing immature neuron marker βIII-tubulin could be detected in these prolonged cultured MSCs. After serum deprivation and growth factor supplement, these nestin-positive cells could express neuron-like morphology and neuron-specific markers NF-H, βIII-tubulin, tau, and neurotransmitter GABA. In contrast, the MSCs without prolonged culture didn't show neuronal morphology nor neuronal markers even after serum withdrawal and growth factors stimulation. These results demonstrated that neural precursors could be obtained from long-term cultured MSCs, and suggested that MSCs should be useful as a potential source for treatment of neurological disease.

【 授权许可】

Unknown