Enzyme-Linked Immunosorbent Assay for the Detection of Canine Leptospira Antibodies Using Recombinant OmpL1 Protein

Journal of Veterinary Medical Science

Atsuhiko HASEGAWA¹  Megumi MATSUUCHI²  Masaru OKUDA²  Takafumi ONISHI²  Yoshiko SAKAI²  Tatsuo OIKAWA²  Kazuhito ITAMOTO²  Hiroyuki IWATA³  Hisashi INOKUMA²  Rui KANO¹  Malaika WATANABE² 

[1] Department of Pathobiology, Nihon University School of Veterinary Medicine;Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University;Laboratory of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University

关键词: canine;    ELISA;    leptospirosis;    OmpL1;    recombinant;   

DOI  :  10.1292/jvms.67.249

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Cited-By(6)OmpL1 is a 31-kDa outer membrane protein characterized in 1993 and known to be expressed only in pathogenic Leptospira spp. Recombinant OmpL1 (GST-rOmpL1) was expressed for use as an ELISA antigen for the detection of anti-Leptospira antibodies. In immunoblot analysis, the protein reacted with sera of dogs infected with three different serotypes of Leptospira interrogans, while did not react with sera of dogs both uninfected negative controls and infected with Borrelia burgdorferi, which is closely related to Leptospira spp. Moreover, in ELISA using GST-rOmpL1, the optical density (O.D.) values from the positive controls were very high (1.125 ± 0.549). In contrast, the O.D. values from clinically healthy dogs and dogs with diseases other than leptospirosis were very low (0.109 ± 0.046 and 0.089 ± 0.046, respectively). These data suggest that the detection of anti-Leptospira antibodies by ELISA using the GST-rOmpL1 protein can be applied for diagnosis of canine leptospirosis.

【 授权许可】

Unknown