Decreased Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinase in the Kidneys of Hereditary Nephrotic (ICGN) Mice

Journal of Veterinary Medical Science

Sayuri ANANN²  Junichiro MATSUDA¹  Noboru MANABE²  Kaoru TAKANO¹  Yoshie YAMAMOTO¹  Yasufumi GOTO²  Atsuo OGURA³  Kozue UCHIO-YAMADA¹ 

[1] Department of Veterinary Sciences, National Institute of Infectious Diseases;Research Unit for Animal Life Sciences, Animal Resource Science Center, Department of Veterinary Medical Sciences, The University of Tokyo;RIKEN Bioresource Center

关键词: extracellular matrix (ECM);    ICR-derived glomerulonephritis (ICGN) mouse;    matrix metalloproteinase (MMP);    renal fibrosis;    tissue inhibitor of metalloproteinase (TIMP);   

DOI  :  10.1292/jvms.67.35

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(5)Matrix metalloporoteinases (MMPs), which are dominantly regulated by tissue inhibitors of metalloproteinase (TIMPs), play important roles in extracellular matrix (ECM) degradation and are involved in the progression of kidney diseases. In glomeruli and tubulointerstitum of hereditary nephrotic (ICR-derived glomerulonephritis: ICGN) mouse kidneys, hyper-accumulation of ECM components occurred, and MMP activity decreased. In the present study, because lower levels of MMP activity may contribute to the progression of renal fibrosis in ICGN mice, Western blotting analysis and immunohistochemical staining for MMPs and TIMPs were performed to verify the expression levels of these proteins. Levels of MMP-2, MMP-9, MT1-MMP, TIMP-1 and TIMP-2 in the kidneys were decreased in ICGN mice in comparison with normal ICR mice. These results indicate that small amounts and low levels of activity of MMPs cause the progression of renal fibrosis in ICGN mice.

【 授权许可】

Unknown