Influence of different methods of collection from the canine epididymides onpost-thaw caudal epididymal sperm quality

Journal of Veterinary Medical Science

Tatsuya HORI¹  Masanori KOBAYASHI¹  Eiichi KAWAKAMI¹  Tetsuya ATAGO¹ 

[1] Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1�?7�?1 Kyonan-cho, Musashino-shi, Tokyo 180�?8602, Japan

关键词: canine;    collection method;    epididymal sperm;    frozen semen;   

DOI  :  10.1292/jvms.14-0421

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(2)Canine epididymal sperm was collected from the cauda epididymis using 2different methods (flushing and mincing) to compare the qualities (the percentage ofprogressively motile, viable, morphologically abnormal, immature and intact acrosomes)before and after freezing and thawing. No significant difference was noted in the qualityof the cauda epididymal sperm immediately after collection and after freezing-thawingbetween the collection methods, although the mean levels of sperm quality with theflushing method were slightly better than that of the mincing method. The flushing methodis simple and free of blood contamination, although the vas deferens was too small to beperfused in only 1 dog, and our results suggest that the flushing method is preferable tothe mincing method for collecting sperm from the canine cauda epididymis.

【 授权许可】

Unknown